Combi Flash 7650 Series Manual de usuario descargar pdf (Pagina 130)

CombiFlash Torrent® User Manual

4-50

module, first open the MANUAL CONTROL [4.3.9] window by

selecting the Tools>Manual Control menu command.

4.4.10.1 Pumping Solvents

To pump either solvent, select the solvent from the Solvent A

or Solvent B lists. Then, click the Prime A or Prime B buttons

to pump the selected solvent. To pump a mixture or Solvent A

and B, adjust the Percentage Solvent B setting and then click

the Prime __% B button. When finished, click the Stop button.

You can control the flow rate by adjusting the Flow Rate

mL/min setting.

By default, the module pumps the solvent directly to the waste

port. You can change the fluid path by selecting a different

Valve Position option (through column or through cartridge

and column). For valve positions other than Direct To Waste,

you can pump the solvent into a collection tube. To do so, select

the Pump Into Tube # option and then select which tube

(“next” or specific tube number).

4.4.10.2 Air Purging

You can purge the solid sample cartridge and column path

with air. To air purge a path, click the Air Purge Solid Sample

or Air Purge Column buttons. Click the Stop button to halt the

air purge.

4.4.10.3 Raw Lamp Energy

The right side of the Manual Control window shows a Raw

Lamp Energy gauge. This provides an indication of the UV

light measured by the optical detector at 254 nm. High lamp

energy (green) means that the flow cell easily passes through

a sufficient UV light source. Lower lamp energy (yellow or red)

means that either the light source is weak or that the flow cell

is obstructed.

• Green – lamp energy is sufficient to detect peaks up to

3.0 Absorbance Units.

• Yellow – lamp energy is partially obstructed and the

peak detector performance might be impaired. The

module can be operated although the absorbance trace

might appear noisy. Yellow might be a normal indication

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Combi Flash 7650 Series Manual de usuario Pagina 130