Combi Flash 7650 Series Manual de usuario descargar pdf (Pagina 117)

Section 4 User Help Reference

4-37

If you will transfer the sample mixture directly from a

container, attach the tubing to the pump inlet. Next,

place the weighted end into the mixture container.

c. Place the On/Off (I/O) switch in the On position.

Adjust the pump speed using the control knob.

d. When the desired sample volume has been loaded,

place the On/Off (I/O) switch in the Off position.

e. Some of the sample mixture will remain in the tubing

before the automated injection valve. In most

applications, pump a chase solution to force the

sample mixture through the injection valve. To do so,

repeat steps b through d using a suitable chase

solvent.

You may allow the module to proceed with the run while

monitoring the progress on the PeakTrak Main window. If

desired, gradient parameters may be modified during the run.

(See R

EAL-TIME GRADIENT EDITING [4.4.6].)

As the run progresses, the absorbance trace is drawn on the

Gradient Plot area. The purification continues until the end of

the run defined by the run length setting.

Note: Solvent may be under pressure. Do not remove a

column or solid load cartridge until the pressure gauge on the

touch screen to return to 0 psi.

Note: After use, pump clean solvent to remove sample

residue.

See Also:

EDISEP RF COLUMN SELECTION GUIDE [ON-LINE]

SOLVENT SELECTION TABLE [ON-LINE]

EDITING A METHOD [4.4.2]

EDITING A DEFAULT METHOD [4.4.4]

BYPASSING THE SOLID LOAD CARTRIDGE [4.4.8]

4.4.2 Editing a method

Editing a method allows you to tailor the operation of the

CombiFlash Torrent module to best separate or purify the

compounds of interest.

PeakTrak has two types of method settings — basic and

advanced.

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Combi Flash 7650 Series Manual de usuario Pagina 117