Combi Flash 7650 Series Manual de usuario descargar pdf (Pagina 127)

Section 4 User Help Reference

4-47

TLC, wait at least the time established as T. After this time

has elapsed it is likely that the desired compound has come

off the cartridge and you can safely click the Cartridge

Bypass button.

For example, a TLC plate run at 30%B revealed that the last

desired compound had an Rf factor of 0.35. An empty 25-gram

size solid load cartridge was filled with sample adsorbed onto

10 grams of silica, and then placed on the system. The

programmed flow rate was 60 mL/min. During the run, the

flow rate dropped to 30 mL/min. The equation is then:

T = 45 mL / (0.35 * 30 mL/min) = 4.3 minutes

In this example, the Cartridge Bypass feature was used 4.3

minutes after the solvent gradient reached 30% B.

The calculation is conservative, but allows you to minimize the

time needed to keep the solid load cartridge in the fluid path.

4.4.9 Viewing runs

After completing a run, the PeakTrak Main window is used to

display all collected run data. You can also open previous runs

to view the chromatogram and the peak/tube locations. To

open a previous run:

1. From the main window, select File>Open, or click the Open

button. The F

ILES [4.3.5] window is displayed.

2.Choose a Run file and click the Open button.

3.PeakTrak displays the run in the M

AIN WINDOW [4.3.1].

The PeakTrak Main window will include:

• Rack and tube information — The left pane of the

window lists the current rack, a map for that rack, and a

table that lists the peaks and their corresponding tube

numbers. If the window is currently displaying the

collection parameters, click the Rack View button to

view this information.

• Method parameters — Click the Display Method

button to view a summary of the peak detection and

collection settings for the run.

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Combi Flash 7650 Series Manual de usuario Pagina 127