Combi Flash EX Especificaciones descargar pdf (Pagina 41)

2. Click on the [Instrument Parameters] tab and select [Absorbance] as the

Measuring Mode. Click [OK] and the Photometer status bar will display

“Slewing” and then the current wavelength value of 550 nm.

B. To Prepare a Powder Sample

1. Place a cuvette of deionized water into the sample compartment of the

spectrophotometer.

2. Click [Auto Zero] on the Photometer Button bar. (This step will zero the

Photometer unit at a designated wavelength, i.e. 550 nm, and correct small

changes, such as drift due to thermal effects.)

3. After the [Auto Zero] completes, the absorbance reading in the Photometer Status

bar should be zero. Remove the cuvette from the sample compartment.

4. Add a small amount of EDTA to the cuvette that contains deionized water.

5. Shake or stir the sample and place it into the sample compartment of the

spectrophotometer.

6. Ensure that the [Photometer Status] is checked in the View pull down menu and

that the initial absorbance value is close to 1.5 on the Photometer Status bar.

When the absorbance value is too low, add more EDTA. When too high, dilute

with deionized water.

C. To Perform a Time Course Reading

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Combi Flash EX Especificaciones Pagina 41