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Spectrofluorophotometer Standard Operating Procedure
Model:
Serial Number:
For the Panoroma Fluorescence 2.1 software:
1) Hit the white switch to turn on the spectrofluorophotometer. Do not touch the XE lamp
switch (black). Listen for the clicking sounds. Once the instrument is on, the power
indicator lamp (front face) will light up.
2) Wait for 15 minutes.
3) Open software “Panoroma Fluorescence 2.1”.
4) Insert cuvette into sample holder (position 1).
5) Under “RF5301”tab, click on “2D emission”.
6) Set parameters.
7) Hit “measure”
After data aqusition: turn off instrument (white switch), then close software.
For the RF5301-PC software:
Turn the xenon power switch ON. Then turn ON power to the instrument. Listen for a clicking
sound. The lamp will automatically light. Verify the power display lamp on the front of the
instrument is ON.
If the xenon lamp does not light or if there is no clicking sound or if the clicking sound continues
without the xenon lamp lighting, switch power to OFF immediately.
Open software
Initialization procedure will begin. Requires about 30 seconds.
Application window will appear.
SPECTRUM SCAN
Acquire Mode—Spectrum
Configure—Parameters
Set the following:
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